Composite

Part:BBa_K3893015:Design

Designed by: Camila Gallegos   Group: iGEM21_Ecuador   (2021-10-21)


Down part of cassette for dsRNA constitutive production 4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 798
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 798
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 798
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 128


Design Notes

In order to use, test and produce the dsRNA we design before, we created a plasmid backbone with an insert that is able to receive different sequences of dsRNA with a BglII and KpnI digestion.

With this in mind, we face some shortcomings: first, it is a short fragment, with many sequence repeats, which makes it difficult for synthesis. Second, we want this insert to behave as a Biobrick compatible once it has the desired dsRNA sequence cloned inside.

It isn't compatible with RFC10 Assembly. Because the XbaI and KpnI restriction sites are needed in the middle of the sequence for the assembly system.

Source

Set of biobricks